Ion Metallic Affinity Chromatography and Purification of Bacterial Toxin

The history of the scientific development of the Chromatography as a chemical separation tool cannot be considered ancient, as the proper establishment of Chemistry as a science is considered modern when compared to the other sciences developed before it. The first literary description of the Chromatography separation technique development is attributed to the works or Mikhail Tswet in 1890 by some authors, where the chemical separation of plant extracts was conducted inside laboratory and first established, permitting the chemical separation of plant chemical substances according to their chemical characteristics of polarity and chemical affinity to the stationary phase. At that time, due to the color attributed to the plant extracts separated experimentally, the technique was named as “chromatography”, where chromato means color and graphy means registering or writing. Although the many chemical substances and bio molecules that can be separated by this technique have no color, the name was kept and the technique recognized as one of the most efficient and applicable for laboratory purification and analytical processes.